Direct PCR detection of foot-and-mouth disease virus.
J Virol Methods
; 47(3): 345-9, 1994 May.
Article
em En
| MEDLINE
| ID: mdl-8071421
ABSTRACT
A PCR assay for the detection and characterization of foot-and-mouth disease virus was developed. The procedure allows RT-PCR amplification following direct adsorption of viral suspensions to microtiter plates, avoiding previous steps of phenol-extraction or heating. Using this procedure, FMDV-specific (based on 3D gene sequences), as well as serotype-specific (based on VP1 gene sequences) amplification were achieved for viral samples of serotypes A, O and C, either from cell culture supernatants or from lesions of infected animals. The assay allowed detection of around 15 PFU, being 500-fold more sensitive than a conventional indirect ELISA. This new method constitutes a simple, rapid and efficient alternative for the diagnosis and characterization of FMDV by PCR.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Aphthovirus
/
Febre Aftosa
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Virol Methods
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Espanha