[Ala4]surfactin, a novel isoform from Bacillus subtilis studied by mass and NMR spectroscopies.
Eur J Biochem
; 224(1): 89-96, 1994 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-8076655
ABSTRACT
When Bacillus subtilis S 499 was grown on a culture medium containing L-alanine as nitrogen source, a mixture of surfactins was obtained. Suitable chromatographic conditions allowed the separation of isoforms. Among these compounds, a new variant of surfactin was isolated and its structure was established by chemical and spectrometric methods, especially by NMR spectrometry. It contains a peptide sequence which differs from that of standard surfactin by the replacement of the L-valine residue by L-alanine residue in position 4. The folding mode of [Ala4]surfactin as deduced from NMR results was compared with that of standard surfactin and the structure/properties relationship issuing from the study of this new isoform is discussed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos Cíclicos
/
Bacillus subtilis
/
Proteínas de Bactérias
Idioma:
En
Revista:
Eur J Biochem
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
França