Purification and characterization of a mitochondrial DNA-binding protein that binds to double-stranded and single-stranded sequences of Paracentrotus lividus mitochondrial DNA.
Curr Genet
; 25(4): 350-6, 1994 Apr.
Article
em En
| MEDLINE
| ID: mdl-8082178
ABSTRACT
A mitochondrial protein, able to specifically bind two double-stranded homologous sequences of sea-urchin mitochondrial DNA, has been partially purified from Paracentrotus lividus eggs. This protein, present at a low concentration, is a polypeptide of 40 kDa. One of the binding sequences, located in the main non-coding region, contains the replication origin of the mitochondrial DNA H-strand. By a combination of band-shift, DNase footprinting, and modification interference analyses with homologous and heterologous probes we identified YCYYATCAN(A/T)RC as the minimum sequence required for the binding. The protein also shows a single-stranded DNA-binding activity, as it is able to specifically interact with one of the strands of the binding sites. These features are consistent with a function of the protein in the modulation of sea-urchin mitochondrial DNA replication during the development stages.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ouriços-do-Mar
/
Transcrição Gênica
/
DNA Mitocondrial
/
DNA de Cadeia Simples
/
Proteínas de Ligação a DNA
/
Replicação do DNA
Limite:
Animals
Idioma:
En
Revista:
Curr Genet
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Itália