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Wild-type human p53 activates the human epidermal growth factor receptor promoter.
Deb, S P; Muñoz, R M; Brown, D R; Subler, M A; Deb, S.
Afiliação
  • Deb SP; Department of Microbiology, University of Texas Health Science Center at San Antonio 78284.
Oncogene ; 9(5): 1341-9, 1994 May.
Article em En | MEDLINE | ID: mdl-8152794
We show that wild-type human p53 transactivates the human epidermal growth factor receptor (EGFR) promoter in vivo in a dose-dependent manner, implicating p53 in promotion of cell proliferation. This activation is sensitive to the expression of cellular oncoprotein MDM2 and human papillomavirus type 18 (HPV-18) E6 protein. The p53 response element is localized within -15 and -569 of the promoter. The EGFR promoter does not have a TATA box, and has low activity in Saos-2 cells in the absence of p53. Results from our in vivo transient transfection assays suggest that p53-binding sites, without any other known promoter element, can act as bidirectional promoters in the presence of wild-type p53. Gel retardation analyses suggest that p53 may serve to nucleate TBP on a promoter. We propose that p53 successfully nucleates the transcription complex, possibly via direct interaction with TFIID, and activates the EGFR promoter.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Genes p53 / Regiões Promotoras Genéticas / Receptores ErbB Limite: Humans Idioma: En Revista: Oncogene Assunto da revista: BIOLOGIA MOLECULAR / NEOPLASIAS Ano de publicação: 1994 Tipo de documento: Article País de publicação: Reino Unido
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Genes p53 / Regiões Promotoras Genéticas / Receptores ErbB Limite: Humans Idioma: En Revista: Oncogene Assunto da revista: BIOLOGIA MOLECULAR / NEOPLASIAS Ano de publicação: 1994 Tipo de documento: Article País de publicação: Reino Unido