Replication and multiplication of hepatitis C virus genome in human foetal liver cells.
Res Virol
; 144(4): 275-9, 1993.
Article
em En
| MEDLINE
| ID: mdl-8210708
ABSTRACT
The ability to obtain primary long-term cultures of human foetal hepatocytes maintaining liver differentiation characteristics in serum-free medium prompted us to test their susceptibility to hepatitis C virus infection. Using PCR, we detected the presence of the HCV RNA-positive strand in the supernatants and in the cells of the virus-infected hepatocyte cultures, at various times post-infection. Evidence of effective virus genome replication and multiplication was also based on the time-dependent appearance of the putative HCV RNA-negative strand, the detection of virus replicative intermediates and an increase in HCV genomic templates in the HCV-infected cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hepacivirus
/
Fígado
Limite:
Humans
Idioma:
En
Revista:
Res Virol
Assunto da revista:
VIROLOGIA
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Itália