Human intestinal alkaline phosphatase-binding IgG in patients with severe bacterial infections.
Clin Exp Immunol
; 95(1): 98-102, 1994 Jan.
Article
em En
| MEDLINE
| ID: mdl-8287614
ABSTRACT
Patterns of alkaline phosphatase (AP)-binding proteins were observed in the alkaline pH range of 6.5-9.5 upon isoelectric focusing and blotting of serum from patients with inflammatory diseases. After isolation using affinity chromatography on protein A or immunoaffinity chromatography on AP coupled to cyanogen bromide (CNBr)-activated Sepharose, the AP-binding protein was identified as IgG on Western blots and in ELISA using human IgG-specific antibodies. It was shown that this IgG binds to AP from both calf (bovine) and human intestine. However, it binds neither to the human liver-bone-kidney (LBK) isoform nor to bacterial AP. Moderate reaction was observed with human placental AP. Comparing patients with various diagnoses (n = 284), AP-binding antibodies were mainly found in severe bacterial infections. They were not detected in serum from healthy blood donors (n = 300). The presence of AP-binding IgG was independent of the infected organ and the bacterial species causing infection. This antibody may be useful for discriminating bacterial from viral infection and for indicating severe bacterial inflammation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções Bacterianas
/
Imunoglobulina G
/
Proteínas de Transporte
/
Fosfatase Alcalina
/
Intestinos
Limite:
Humans
Idioma:
En
Revista:
Clin Exp Immunol
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Alemanha