Development and characterization of a rainbow trout liver cell line expressing cytochrome P450-dependent monooxygenase activity.
Cell Biol Toxicol
; 9(3): 279-94, 1993.
Article
em En
| MEDLINE
| ID: mdl-8299006
ABSTRACT
A cell line, RTL-W1, has been developed from the normal liver of an adult rainbow trout by proteolytic dissociation of liver fragments. RTL-W1 can be grown routinely in the basal medium, L-15, supplemented with 5% fetal bovine serum. In this medium, the cells have been passaged approximately 100 times over an 8-year period. The cells do not form colonies or grow in soft agar. The cultures are heteroploid. The cell shape was predominantly polygonal or epithelial-like, but as cultures became confluent, bipolar or fibroblast-like cells appeared. Among the prominent ultrastructural features of RTL-W1 were distended endoplasmic reticulum and desmosomes. Benzo[a]pyrene was cytotoxic to RTL-W1. Activity for the enzyme, 7-ethoxyresorufin O-deethylase (EROD), which is a measure of the cytochrome P4501A1 protein, increased dramatically in RTL-W1 upon their exposure to increasing concentrations of either beta-naphthoflavone (BNF) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). With these properties, RTL-W1 should be useful for studying the expression of the cytochrome P450 enzymes and as a tool for assessing the toxic potency of environmental contaminants.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxigenases
/
Oncorhynchus mykiss
/
Sistema Enzimático do Citocromo P-450
/
Fígado
Limite:
Animals
Idioma:
En
Revista:
Cell Biol Toxicol
Assunto da revista:
TOXICOLOGIA
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Canadá