Localization of xanthine dehydrogenase mRNA in horse skeletal muscle by in situ hybridization with digoxigenin-labelled probe.
Biochem J
; 292 ( Pt 3): 639-41, 1993 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-8317993
ABSTRACT
In situ hybridization was used to localize xanthine dehydrogenase (XDH) mRNA in horse skeletal muscle. Capillary endothelial cells were found to express XDH, but muscle cells did not give any signal. The digoxigenin-labelled probe was produced by PCR with primers based on the cDNA sequence of mouse XDH and horse lung cDNAs. A 4.3 kb mRNA was detected in a Northern blot.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Xantina Desidrogenase
/
RNA Mensageiro
/
Músculos
Limite:
Animals
Idioma:
En
Revista:
Biochem J
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Finlândia