Structure of the promoter region of the gene encoding cytochrome c oxidase subunit V in Dictyostelium.
Eur J Biochem
; 211(3): 411-4, 1993 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-8382151
ABSTRACT
A cDNA for the nuclear-encoded subunit V of Dictyostelium discoideum cytochrome-c oxidase was used as a probe to screen a genomic library and isolate the complete gene. Primer-extension analysis revealed two transcription start sites located 32 and 39 nucleotides upstream of the translation initiation codon. The chloramphenicol acetyltransferase assay in transient and stable Dictyostelium transformants indicated that the 400-bp dT-rich segment 5' to the transcription start sites retained promoter activity. This region contains an octanucleotide sequence similar to the yeast HAP2/3/4 responsive element.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Regiões Promotoras Genéticas
/
Complexo IV da Cadeia de Transporte de Elétrons
/
Dictyostelium
/
Genes Fúngicos
Limite:
Animals
Idioma:
En
Revista:
Eur J Biochem
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Itália