Peripheral B cell maturation. II. Heat-stable antigen(hi) splenic B cells are an immature developmental intermediate in the production of long-lived marrow-derived B cells.
J Immunol
; 151(9): 4431-44, 1993 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-8409411
ABSTRACT
HSAhi B cells comprise 5 to 10% of adult mouse splenic B cells and are phenotypically and functionally immature. To assess their origin and relationship to mature, heat-stable Ag (HSA)lo B cells, we determined HSA and surface IgD phenotype among splenic B cells throughout development, as well as during reconstitution of lethally irradiated adults given adult B-depleted bone marrow. In each case, HSAhi splenic B cells predominate during the earliest stages of B cell genesis. Furthermore, 5-bromo-2'-deoxyuridine labeling experiments indicate rapid turnover within both the marrow and peripheral HSAhi pools, and adoptive transfer studies show that peripheral HSAhi splenic B cells differentiate to HSAlo within 4 days. Finally, splenic HSAhi B cells reconstitute both primary and memory humoral responses. Together, these data indicate that splenic B cells in the HSAhi subset are an intermediate maturational stage in the adult periphery.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Baço
/
Células da Medula Óssea
/
Células-Tronco Hematopoéticas
/
Linfócitos B
/
Antígenos de Diferenciação
Limite:
Animals
Idioma:
En
Revista:
J Immunol
Ano de publicação:
1993
Tipo de documento:
Article