A modification of an affinity procedure for purification of human C1- inhibitor that provides a homogeneous stable preparation.
J Immunol Methods
; 157(1-2): 101-4, 1993 Jan 04.
Article
em En
| MEDLINE
| ID: mdl-8423351
ABSTRACT
Human C1- inhibitor can be rapidly purified by the affinity chromatography procedure described by Pilatte and his associates (1989), but the inhibitor so purified breaks down during storage or is in a cleaved form when initially purified. By adding an ion-exchange chromatography procedure after the affinity chromatography, a stable, single species of C1- inhibitor molecules is obtained. It is likely that serine proteinases in trace amounts, which may be complexed with some of the C1- inhibitor, are removed during the ion-exchange procedure. This procedure provides a highly purified and useful preparation of C1- inhibitor.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Inativadoras do Complemento 1
Limite:
Humans
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
1993
Tipo de documento:
Article