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Expression of silver-stained nucleolar organizer regions is coupled to cell cycle in rat thymic cells.
Carbajo, S; Orfao, A; Vicente-Villardón, J L; Carbajo-Pérez, E.
Afiliação
  • Carbajo S; Department of Human Anatomy and Histology, Universidad de Salamanca, Spain.
Cytometry ; 14(1): 46-52, 1993.
Article em En | MEDLINE | ID: mdl-8432202
To directly analyze the relationship between the expression of silver-stained nucleolar organizer regions (AgNORs) and cell proliferation, thymic cells from newborn rats were separately sorted at the G0-G1-phase and early-mid and late-mid S-phase of the cell cycle according to their DNA content. Different AgNOR-derived parameters (mean area and numbers of AgNORs per cell and mean AgNOR-particle area) were evaluated after silver staining of cytospins. A linear correlation was observed between the mean area and numbers of AgNORs per cell, both parameters increasing progressively from G0-G1-phase to early-mid and late-mid S-phase. An increase of the mean AgNOR-particle area was also seen between G0-G1 and S-phase but this was not significant along the S-phase. A bias on the selection of S-phase cells linked to intrathymic maturation can be ruled out as S-phase cells labeled with bromodeoxyuridine were found throughout the thymus, and, moreover, the analysis of the frequency distribution of nuclear area did not show a bimodal pattern. It is concluded that the expression of AgNORs--evaluated as AgNOR area/cell or AgNOR numbers/cell--is causally or indirectly coupled to DNA synthesis and, thus, AgNORs can be considered as a cell proliferation marker.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Timo / Ciclo Celular / Região Organizadora do Nucléolo Limite: Animals Idioma: En Revista: Cytometry Ano de publicação: 1993 Tipo de documento: Article País de afiliação: Espanha País de publicação: Estados Unidos
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Timo / Ciclo Celular / Região Organizadora do Nucléolo Limite: Animals Idioma: En Revista: Cytometry Ano de publicação: 1993 Tipo de documento: Article País de afiliação: Espanha País de publicação: Estados Unidos