Synthesis of [35S]thiophosphoryl adenylic acid, utilizing a general procedure for [35S]thiophosphoryl chloride production.
Anal Biochem
; 209(1): 143-9, 1993 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-8465947
ABSTRACT
Elemental [35S]sulfur was shown to equilibrate with the sulfur of thiophosphoryl chloride when these materials are heated together. This isotopic exchange reaction is the basis of a convenient, microscale synthesis of high specific activity [35S]PSCl3. [35S]Thiophosphoryl chloride is otherwise not commercially available except through custom synthesis. The labeled thiophosphoryl chloride was used in a novel procedure for the preparation of [35S]adenosine 5'-phosphorothioate. This isotopic exchange method should find wide application in the synthesis of many radiolabeled thiophosphoryl esters which utilize PSCl3 as the source of the thiophosphoryl group.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fósforo
/
Radioisótopos de Enxofre
/
Tionucleotídeos
/
Monofosfato de Adenosina
/
Cloretos
/
Compostos de Fósforo
/
Marcação por Isótopo
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
1993
Tipo de documento:
Article