Detection of antibodies to acetaldehyde-albumin conjugates in alcoholic liver disease.
Alcohol Alcohol
; 28(1): 11-6, 1993 Jan.
Article
em En
| MEDLINE
| ID: mdl-8471080
ABSTRACT
The principal metabolite of ethanol, acetaldehyde, conjugates with various proteins that form antibody-inducing neo-antigens. We have analysed sera from patients with biopsy-proven alcoholic liver disease (hepatitis = 10, cirrhosis = 11, steatosis = 3) and controls = 19 (normal teetotallers and 6 non-alcoholic liver disease). Sera were examined with an enzyme-linked immunoabsorbent assay (ELISA) for antibodies binding preferentially to an acetaldehyde-albumin conjugate. Reactive sera from alcohol misusers were then purified using an amino-hexyl Sepharose affinity column. Antibodies binding to the acetaldehyde-albumin epitopes were significantly raised (P < 0.005) in all groups of alcohol misusers, and were present in greatest titre in those with alcoholic hepatitis. These antibodies were successfully purified using the gel affinity column. We conclude that alcohol misusers have significant titres of antibodies reacting to the acetaldehyde-albumin complex. The role of these antibodies remains nuclear, but may be related to the initiation of an inflammatory response and tissue damage following ethanol consumption.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Autoanticorpos
/
Autoantígenos
/
Proteínas Sanguíneas
/
Hepatopatias Alcoólicas
/
Acetaldeído
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Alcohol Alcohol
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Reino Unido