Immunoactive iso-ANP/BNP in plasma, tissues and atrial granules of the rat.
Regul Pept
; 44(1): 1-9, 1993 Mar 05.
Article
em En
| MEDLINE
| ID: mdl-8484014
ABSTRACT
We have developed a specific radioimmunoassay (RIA) for iso-rANP(1-45)/rBNP(51-95) using antiserum produced against a peptide comprise of the first 20 amino acid sequence of this cardiac hormone. Using this RIA, we found that atria contained the highest amount of iso-rANP(1-45) (567.37 pmol/g) which is about 140-fold higher than ventricles (4.32 pmol/g). With the exception of the lung and kidney, all other tissues had negligible amounts. The plasma level was 1.4 fmol/ml and the only molecular form found was the 45 amino acid form. HPLC analysis of extracts of isolated, purified atrial granules revealed that, like atrial natriuretic peptide (ANP), iso-rANP/rBNP is also stored in these granules. However, while ANP is stored predominantly as pro-ANP(1-126) and cleaved during or after release, iso-ANP/BNP is stored as the 45 amino acid form and may be processed prior to storage in the granules.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fator Natriurético Atrial
/
Grânulos Citoplasmáticos
/
Miocárdio
/
Proteínas do Tecido Nervoso
Limite:
Animals
Idioma:
En
Revista:
Regul Pept
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Canadá