Ultrastructural study of the spermatogenesis and mature spermatozoa of Dicrocoelium dendriticum (Plathelminthes, Digenea).

ABSTRACT

The spermatogenesis and spermatozoon of Dicrocoelium dendriticum were studied by transmission electron microscopy. Peripheric accessory cells project between germ cells. Each spermatogonium gives rise to 32 spermatozoa. The stages in spermiogenesis include development of the zone of differentiation, appearance of the intercentriolar body flanked by two centrioles from each of which a free axoneme and a striated rootlet grow, outgrowth of the differentiation zone to form the median cytoplasmic process and migration of the nucleus and mitochondria into it, and rotation of the flagella and subsequent proximodistal fusion of the three projections to form a monopartite spermatozoon. The spermatozoon possess two incorporated axonemes with the "9 + 1" pattern typical of those in trepaxonematid plathelminths. beta-Glycogen particles accumulate in the spermatozoa after they have separated from the cytophore as revealed by Thiery's method. This study confirms in a further family, Dicrocoeliidae, the constant pattern of spermiogenesis and spermatozoon structure in Digenea.