Monoclonal antibodies against human hemopexin and their application.
J Immunol Methods
; 161(2): 265-8, 1993 May 26.
Article
em En
| MEDLINE
| ID: mdl-8505555
ABSTRACT
Six monoclonal antibodies raised against human serum hemopexin have been characterized. The antibodies reacted with serum hemopexin as well as with the isolated protein in apo-form and with heme-protein complexes on immunoblots obtained following both PAGE and SDS-PAGE. In the case of PAGE blots 1 ng of hemopexin could be detected using a streptavidin-AP detection system. ELISA procedures employing two different pairs of monoclonal antibodies gave working ranges of 0.3-3 mg/l and 10-100 micrograms/l respectively.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hemopexina
/
Anticorpos Monoclonais
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
República Tcheca