Molecular phylogeny of the fungi of the Iceman's grass clothing.
Curr Genet
; 28(3): 289-97, 1995 Aug.
Article
em En
| MEDLINE
| ID: mdl-8529276
ABSTRACT
To investigate the origin of the fungal hyphae that cover the grass clothing (cloak, boots) found near the neolithic mummy known as the Tyrolean Iceman, two radiocarbon-dated samples of grass were submitted to DNA extraction. The DNA was then PCR amplified using, respectively, primers specific for the region containing the internal transcribed spacers and the 5.8s rDNA (ITS), and primers specific for an approximately 600-bp long fragment of the nuclear small-subunit ribosomal DNA (SSU rDNA) repeat units of eukaryotes. The amplification products were cloned and sequenced. Sequence analysis of 20 individual ITS clones and of ten SSU rDNA clones indicated that three types of fungal DNA can be extracted from the grass. Phylogenetic analyses, using 5.8s and SSU rDNA fungal reference sequences from EMBL and GenBank databases, suggest that the DNAs come, respectively, from a psychrophilic basidiomycetous yeast, phylogenetically close to Leucosporidium scottii, and from two ascomycetes, one of which is possibly related to the Eurotiales.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ascomicetos
/
Basidiomycota
/
Múmias
/
Vestuário
/
Poaceae
Limite:
Humans
País/Região como assunto:
Europa
Idioma:
En
Revista:
Curr Genet
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Itália