Lack of correlation between glutamate-induced depletion of ATP and neuronal death in primary cultures of cerebellum.
Brain Res
; 695(2): 146-50, 1995 Oct 16.
Article
em En
| MEDLINE
| ID: mdl-8556324
ABSTRACT
The aim of this work was to identify, using primary cultures of cerebellar neurons, the receptors involved in glutamate-induced depletion of ATP and to assess whether there is a correlation between glutamate-induced ATP depletion and neuronal death. Glutamate induced a rapid depletion of ATP (40% decrease at 5 min). After 60 min incubation with 1 M glutamate ATP content decreased by 60-70%. Similar effects were induced by glutamate, NMDA and kainate while quisqualate, AMPA or trans-ACPD did not affect significantly ATP content. The EC50 were approximately 6, 25 and 30 microM for glutamate, NMDA and kainate, respectively. DNQX and AP-5, competitive antagonists of kainate and NMDA receptors, respectively, prevented in a dose-dependent manner the glutamate-induced depletion of ATP. These results indicate that glutamate-induced depletion of ATP is mediated by activation of kainate and NMDA receptors. Glutamate-induced neuronal death was prevented by MK-801, calphostin C, H7, carnitine, nitroarginine and W7. However, only MK-801 and W7 prevented glutamate-induced depletion of ATP, while calphostin C, H7, carnitine and nitroarginine did not. This indicates that there is not a direct correlation between ATP depletion and neuronal death.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cerebelo
/
Trifosfato de Adenosina
/
Ácido Glutâmico
/
Neurônios
Limite:
Animals
Idioma:
En
Revista:
Brain Res
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Espanha