Beet necrotic yellow vein virus 42 kDa triple gene block protein binds nucleic acid in vitro.
J Gen Virol
; 77 ( Pt 5): 889-97, 1996 May.
Article
em En
| MEDLINE
| ID: mdl-8609485
ABSTRACT
The triple gene block (TGB) of beet necrotic yellow vein virus RNA 2 is required for cell-to-cell movement of the virus RNA. The protein P42 encoded by the 5'-proximal gene of the TGB has consensus sequence motifs characteristic of an ATP/GTP-dependent helicase. P42 was over-expressed in Escherichia coli and shown to bind both single- and double-stranded RNA and DNA by Northwestern blotting. Site-directed mutagenesis located the nucleic acid-binding domain to the N-terminal 24 amino acids of the protein and a point mutation or deletions in the region of P42 containing the helicase consensus sequences did not affect nucleic acid-binding activity of the immobilized protein. Electrophoretic mobility-shift assays revealed that P42 also binds nucleic acids in solution and that deletion of the N-terminal region inhibits this binding. Mutations in both the N-terminal nucleic acid-binding domain and the helicase domain blocked infection of leaves, indicating that both regions of P42 are important for its activity in vivo.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus de Plantas
/
Vírus de RNA
/
Proteínas Virais
/
DNA
/
RNA
Idioma:
En
Revista:
J Gen Virol
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
França