Induction of immune responses to functional determinants of a cell surface streptococcal antigen.
Immunology
; 87(1): 55-63, 1996 Jan.
Article
em En
| MEDLINE
| ID: mdl-8666436
ABSTRACT
Antibodies directed against the cell surface adhesin, termed streptococcal antigen I/II of Streptococcus mutans can protect against dental caries. Streptococcal antigen I/II (SA I/II) interacts with salivary glycoproteins and promotes adhesion to the tooth surface. Topical application of monoclonal antibodies which recognize a domain within residues 816-1213 (fragment 3) prevents colonization by S. mutans in primates. In this study the immunogenicity and antigenicity of fragment 3 was investigated in five strains of mice. Fragment 3 induced an immune response following immunization with whole cells of S. mutans in all strains of mice. Immunization with recombinant fragment 3 also induced T-cell proliferative and antibody responses both to fragment 3 and to the SA I/II. Antibody responses to the previously defined adhesion determinants (residues 1005-1044) were weak or undetectable. Immunization of three representative strains of mice with a recombinant polypeptide (residues 975-1044) comprising this adhesion epitope and an adjacent T-cell epitope (residues 975-1004) elicited both T- and B-cell responses to the polypeptide and to native SA I/II. The B-cell epitopes overlapped with the adhesion determinant. These findings provide a means of directing immune responses to functional determinants of SA I/II.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Streptococcus mutans
/
Vacinas Bacterianas
/
Vacinas Sintéticas
/
Linfócitos T
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Anticorpos Antibacterianos
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Antígenos de Bactérias
/
Antígenos de Superfície
Limite:
Animals
Idioma:
En
Revista:
Immunology
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Reino Unido