TFIIH functions in regulating transcriptional elongation by RNA polymerase II in Xenopus oocytes.
Mol Cell Biol
; 16(7): 3291-9, 1996 Jul.
Article
em En
| MEDLINE
| ID: mdl-8668144
ABSTRACT
We investigated the role of TFIIH in transcription by RNA polymerase II (pol II) in vivo by microinjection of antibodies against this factor into Xenopus oocytes. Five different antibodies directed against four subunits of TFIIH were tested for effects on transcription of coinjected human immunodeficiency virus type 2 and c-myc templates. Each of these antibodies severely reduced the efficiency of elongation through human immunodeficiency virus type 2 and c-myc terminator elements. In contrast, an anti-TFIIB antibody did not inhibit elongation. Anti-TFIIH antibodies also had a much smaller inhibitory effect on total transcription than did anti-TFIIB or anti-pol II large subunit. Three inhibitors of TFIIH kinase activity, H-7, H-8, and dichlororibofuranosylbenzimidazole (DRB), inhibited elongation similarly to anti-TFIIH antibodies. These results strongly suggest a role for TFIIH in the stimulation of transcriptional elongation in vivo.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oócitos
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Fatores de Transcrição
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Transcrição Gênica
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RNA Polimerase II
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Genes myc
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HIV-2
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Fatores de Transcrição TFII
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Proteínas de Drosophila
Limite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Mol Cell Biol
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Canadá