The moulting hormone ecdysone is able to recognize target elements composed of direct repeats.
Mol Cell Endocrinol
; 113(1): 1-9, 1995 Aug 30.
Article
em En
| MEDLINE
| ID: mdl-8674807
ABSTRACT
In Drosophila melanogaster, three temporally distinct ecdysone-responsive puff sets, the so-called intermoult, early and late puffs, have been described on the salivary gland polytene chromosomes. We have analyzed in detail a DNA segment of the 3C polytene region, from which the originates one of the most prominent intermoult puffs, with the aim of identifying ecdysone response elements (EcREs). Here we report that two putative EcREs of identical sequence are located at this puff site. Interestingly, these elements display a novel structural feature, being composed of directly repeated half-sites. Our results show that the EcR/USP heterodimer known to constitute the ecdysone functional receptor complex is able to bind to and transactivate through target elements composed of directly repeated half-sites. In addition, we show that these elements are also able to bind efficiently USP alone, suggesting that USP and EcR/USP could compete for their binding to DNA.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Sequências Repetitivas de Ácido Nucleico
/
Receptores de Esteroides
/
Drosophila melanogaster
/
Ecdisona
Limite:
Animals
Idioma:
En
Revista:
Mol Cell Endocrinol
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Itália
País de publicação:
IE
/
IRELAND
/
IRLANDA