Heavy-metal-responsive genes in maize: identification and comparison of their expression upon various forms of abiotic stress.
Planta
; 199(1): 1-8, 1996.
Article
em En
| MEDLINE
| ID: mdl-8680303
ABSTRACT
To identify genes involved in defense against heavy-metal stresses, a cDNA library originating from mercuric chloride-treated maize (Zea mays L. cv. INRA 258) leaves was constructed and analysed by differential screening using cDNAs derived from treated and untreated plants. Transcriptionally activated cDNA clones, designated CHEM (chemically-activated), were isolated and characterized. They represent various known proteins, such as glycine-rich proteins, pathogenesis-related proteins, chaperones and membrane proteins. The expression of the genes encoding these proteins was studied in maize subjected to other forms of abiotic stress. Expression of glycine-rich proteins was greatly enhanced by heat stress, and also stimulated by NaCl, polluted rainwater, wounding and cold stress. Pathogenesis-related proteins were strongly induced by ultraviolet light and to a lesser extent by NaCl, polluted rainwater and wounding. Heat-shock protein was mainly induced by heat and cold, and ubiquitin by wounding. Expression of the membrane channel protein was stimulated by heat stress, NaCl, polluted rainwater and ultraviolet-light irradiation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Genes de Plantas
/
Zea mays
/
Cloreto de Mercúrio
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Planta
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
França