In vitro biosynthesis and in vivo processing of the major microneme antigen of Sarcocystis muris cyst merozoites.
Parasitol Res
; 82(5): 468-74, 1996.
Article
em En
| MEDLINE
| ID: mdl-8738288
ABSTRACT
The cDNA clone pSM/1.6 encoding the 26.5-kDa precursor molecule of the 16/17-kDa microneme antigen of Sarcocystis muris cyst merozoites was expressed in a cell-free translation/translocation system to study translocation of the protein across membranes. The antigen was found to be translocated across heterologous endoplasmic reticulum membranes. Translocation was accompanied by cleavage of a signal peptide to create a 23-kDa polypeptide that was completely protected from digestion with proteinase K. Pulse-chase analysis of [35S]-methionine-labeled S. muris cyst merozoites demonstrated that the 16/17-kDa antigen derived from a 23-kDa precursor molecule and that its processing occurred at between a few minutes and 2 h after biosynthesis. This leads to the conclusion that the native microneme antigen is secreted from the parasite cell via the endoplasmic reticulum. Sorting into micronemes might occur during transition through a Golgi-like structure, involving cleavage of the hydrophilic propeptide to create the mature 16/17-kDa protein.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sarcocystis
/
Antígenos de Protozoários
Limite:
Animals
Idioma:
En
Revista:
Parasitol Res
Assunto da revista:
PARASITOLOGIA
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Alemanha