Structural and serological characterisation of two O-specific polysaccharides of Acinetobacter.
Eur J Biochem
; 239(3): 602-10, 1996 Aug 01.
Article
em En
| MEDLINE
| ID: mdl-8774703
ABSTRACT
Extraction of dry bacteria of Acinetobacter strain 34 (DNA group 2) or Acinetobacter strain 108 (DNA group 13) by phenol/water yielded a polymer that was identified by means of serological studies and fatty acid analysis as S-form lipopolysaccharide. Degradation of the lipopolysaccharides of strains 34 and 108 in 1% acetic acid and 5% acetic acid, respectively, and gel-permeation chromatography gave the respective O-antigenic polysaccharides, the structures of which were determined, by compositional analysis and NMR spectroscopy of the polysaccharide, as [Sequence see text] for strain 108, where D-Fucp3NBuOH represents 3-[(R)-3-hydroxybutyramido] -3,6-dideoxy-D-galactose and D-GalpANAc represents 2-acetamido-2-deoxy-D-galacturonic acid. Both structures were specifically recognised in Western blots by polyclonal rabbit antisera and there was no cross-reaction between these two structures.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Acinetobacter
/
Antígenos O
Idioma:
En
Revista:
Eur J Biochem
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Alemanha