Shiga-like toxin purges human lymphoma from bone marrow of severe combined immunodeficient mice.
Blood
; 88(5): 1561-7, 1996 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-8781410
ABSTRACT
Shiga-like toxin-1 (SLT-1) is a bacterial toxin that kills cells by inhibiting protein synthesis. SLT-1 is composed of one cytotoxic A-subunit and five B-subunits that bind to CD77, a cell-surface glycolipid. In the human hematopoietic system, CD77 expression is restricted to a subset of activated B cells and derived cancers. Here we report that SLT-1 treatment of murine bone marrow ex vivo effectively cures severe combined immunodeficient mice of a human B-cell lymphoma xenograft while sparing normal hematopoietic precursor cells. Flow cytometry results using fluorescein isothiocyanate-labeled SLT-1 B-subunit show the high prevalence of expression of SLT-1 receptors (CD77) in human non-Hodgkin's lymphomas, especially follicular lymphomas. These results suggest the use of SLT-1 for the purging of human bone marrow before autologous bone marrow transplant in the case of CD77+ B-cell lymphomas as just one of many possible uses.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Toxinas Bacterianas
/
Triexosilceramidas
/
Medula Óssea
/
Linfoma de Burkitt
/
Transplante de Medula Óssea
/
Purging da Medula Óssea
/
Antineoplásicos
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Blood
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Canadá