Mediation of interleukin-11-dependent biological responses by a soluble form of the interleukin-11 receptor.
Biochem J
; 318 ( Pt 2): 489-95, 1996 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-8809037
ABSTRACT
Interleukin-11 (IL-11) is a polyfunctional cytokine whose biological actions require a specific IL-11 receptor (IL-11R) and the transmembrane transducer gp130. Here we report the production of a soluble form of the murine IL-11R and demonstrate that it interacts with IL-11 ligand with high affinity. The affinity of IL-11 alone for gp130 is below the level of detection, but a complex of IL-11 and soluble IL-11R interacts with gp130 with high affinity. The addition of soluble IL-11R potentiates the effects of exogenous IL-11 in cells that are normally responsive to IL-11. A biological response to IL-11 can be reconstituted in BAF cells transfected with gp130 by addition of IL-11 and soluble IL-11R. These findings show that the cytoplasmic domain of the IL-11R is not required for the biological effects of IL-11 and that a complex of IL-11 and IL-11R mediates signalling by association with gp130.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Interleucina-11
/
Receptores de Interleucina
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Biochem J
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Reino Unido