Evidence for the presence of kinetin in DNA and cell extracts.
FEBS Lett
; 393(2-3): 197-200, 1996 Sep 16.
Article
em En
| MEDLINE
| ID: mdl-8814289
ABSTRACT
In contrast to the current view that kinetin (N6-furfuryladenine) is an unnatural and synthetic compound, we have detected it in commercially available DNA, in freshly extracted cellular DNA from human cells and in plant cell extracts by two independent methods. First, we discovered that N6-furfuryladenine has electrochemical properties that can be applied for monitoring this modified base by a HPLC/UV/EC method. Second, we have confirmed electrochemical assignments by mass-spectrometric analysis. A pathway of kinetin formation is proposed in which the formation of furfural by oxidative damage of the deoxyribose moiety of DNA is followed by its reaction with adenine residues to form N6-furfuryladenine. Since this modification can lead to mutations, the odd DNA base has to be removed by repair enzymes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Adenina
Limite:
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Dinamarca