Testosterone effects on vasotocinergic innervation of sexually dimorphic medial preoptic nucleus and lateral septum during aging in male quail.

ABSTRACT

Vasotocin fibers are known to innervate regions important in the regulation of sexual behavior and neuroendocrine systems in quail. In this experiment, vasotocinergic innervation of the lateral septum and of the sexually dimorphic medial preoptic nucleus was studied during reproductive aging relative to sexual behavior or following testosterone (T). There were 4 groups of male Japanese quail (Coturnix japonica) studied adult reproductive (6 month, n = 4), photoregressed adult (n = 5), old senescent (36 month, n = 4), and old testosterone-treated (n = 5). Immunocytochemistry for vasotocin (VT) was performed on serial sections and quantification of the density of VT-positive fibers was performed by image analysis. Results showed a highly significant decrease in VT-immunocytochemical staining in photoregressed and in old senescent males; whereas T-treatment in old males was associated with recovery of VT-immunocyto-chemical staining, comparable to the adult reproductive male. Previous experiments have shown that T treatment restimulates sexual behavior in senescent males similar to the recovery of sexual behavior in T-treated castrates. These results indicate that the VT system may be associated with the behavioral recovery observed in senescent T-treated males.