Conformational aspects of an uncharged phosphate analogon built in at the branch-point of a DNA four-way junction.
J Mol Biol
; 262(3): 349-57, 1996 Sep 27.
Article
em En
| MEDLINE
| ID: mdl-8845000
ABSTRACT
Electrostatic interactions appear to be important in the conformation and dynamics of DNA four-way junctions. Particularly, the Coulomb repulsion between two approaching phosphate groups at the site of strand exchange is commonly supposed to have a negative influence on the thermodynamic stability of the fully stacked conformation. We synthesized a unimolecular DNA four-way junction containing an uncharged methylene-acetal linkage, -O3'-CH2-O5'-, instead of a regular phosphodiester linkage, -O3'-PO2-O5'-, between two specific residues at the branch-point to examine the effect of charge removal. Complete sequential 1H-NMR assignments of the non-exchangeable base protons and H1'/H2'/H2" sugar protons were obtained with the aid of NOESY and TOCSY experiments. On the basis of the NMR data it is concluded that the stacking arrangement at the branch-point of the modified oligonucleotide is similar to that of the previously studied parent four-way junction. Surprisingly, this is not the stacking arrangement in which the close phosphate-phosphate contact at the site of strand exchange would be absent. Some implications of this novel information regarding the role of the phosphate-phosphate repulsion in four-way junctions are discussed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Conformação de Ácido Nucleico
Idioma:
En
Revista:
J Mol Biol
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Holanda