Concanamycin B, a vacuolar H(+)-ATPase specific inhibitor suppresses bone resorption in vitro.
Biol Pharm Bull
; 19(2): 297-9, 1996 Feb.
Article
em En
| MEDLINE
| ID: mdl-8850326
ABSTRACT
Effects of concanamycin B, a specific inhibitor of the vacuolar type H(+)-ATPase (V-ATPase), on the stimulation of bone resorption induced by parathyroid hormone (PTH) were examined in vitro. Concanamycin B was found to inhibit PTH-stimulated osteoclastic pit formation and to suppress the acidification of vacuolar organelles by V-ATPase in the osteoclasts. PTH-stimulated 45Ca release from prelabelled chick embryonic calvariae was also inhibited by concanamycin B in a dose-dependent manner. These results suggest that osteoclastic acidification of lacunae by V-ATPase plays an essential role in mineral dissolution and degradation of the organic matrix during bone resorption.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vacúolos
/
Reabsorção Óssea
/
ATPases Translocadoras de Prótons
/
Macrolídeos
/
Antibacterianos
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Biol Pharm Bull
Assunto da revista:
BIOQUIMICA
/
FARMACOLOGIA
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Japão