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Mapping amino acids of the measles virus hemagglutinin responsible for receptor (CD46) downregulation.
Bartz, R; Brinckmann, U; Dunster, L M; Rima, B; Ter Meulen, V; Schneider-Schaulies, J.
Afiliação
  • Bartz R; Institut für Virologie und Immunbiologie, Wörzburg, Germany.
Virology ; 224(1): 334-7, 1996 Oct 01.
Article em En | MEDLINE | ID: mdl-8862431
We compared the amino acid sequences of groups of receptor (CD46) downregulating and nondownregulating measles virus (MV) hemagglutinins (Hs) and identified seven group-specific differences as candidates for the mediation of the observed differential effects. Using site-directed mutagenesis, we mutated the chosen amino acids of the H of MV-strain WTF (WTF-H), a nondownregulating H, and Introduced the corresponding amino acids of Edmonston-H (Edm-H), a downregulating H. We identified four amino acids, 211G, 243R, 451V, and 481Y, which influenced the downregulative function when introduced into WTF-H. The double mutation 451V and 481Y in WTF-H led to a degree of CD46 downregulation comparable to that of Edm-H. Conversely, introducing amino acids 451E and 481N into Edm-H resulted in a loss of the downregulative function. These results indicate that these amino acids play a decisive role in the H-CD46 interaction.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Receptores Virais / Glicoproteínas de Membrana / Antígenos CD / Hemaglutininas Virais / Vírus do Sarampo Tipo de estudo: Prognostic_studies Idioma: En Revista: Virology Ano de publicação: 1996 Tipo de documento: Article País de afiliação: Alemanha País de publicação: Estados Unidos
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Receptores Virais / Glicoproteínas de Membrana / Antígenos CD / Hemaglutininas Virais / Vírus do Sarampo Tipo de estudo: Prognostic_studies Idioma: En Revista: Virology Ano de publicação: 1996 Tipo de documento: Article País de afiliação: Alemanha País de publicação: Estados Unidos