A screening procedure for the determination of 13 oral anticoagulants and rodenticides.
J Anal Toxicol
; 20(2): 93-100, 1996.
Article
em En
| MEDLINE
| ID: mdl-8868399
ABSTRACT
A technique for the simultaneous identification and quantitation of 13 hydroxycoumarin and indandione anticoagulant drugs and rodenticides from human serum by reversed-phase liquid chromatography with diode-array detection has been developed. High-performance liquid chromatography was performed using gradient elution with an acetonitrile and phosphate buffer on a Nucleosil ODS column. Ultraviolet spectra from 200 to 400 nm were recorded on-line during the analysis and compared with spectra stored in a library. For the spiked 2 mL of serum, acidic and alkaline liquid-liquid double extraction with diethylether-ether acetate (5050, v/v) was conducted, and recoveries greater than 60% for most compounds were found. The detection limit was approximately 25 or 50 ng/mL for all components except for difethialone and fluindione, for which it was approximately 100 ng/mL. The standard calibration curves were linear from the detection limit to 5000 ng/mL. The within-run precision coefficient of variation (CV) was less than 10%, and the between-run precision CV was less than 20%.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Rodenticidas
/
Anticoagulantes
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Limite:
Humans
Idioma:
En
Revista:
J Anal Toxicol
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
França