Characterization of a nosocomial outbreak involving an epidemic plasmid encoding for TEM-27 in Salmonella enterica subspecies enterica serotype Othmarschen.
J Infect Dis
; 174(5): 1015-20, 1996 Nov.
Article
em En
| MEDLINE
| ID: mdl-8896503
ABSTRACT
A ceftazidime-resistant Salmonella enterica subspecies enterica serotype Othmarschen strain, harboring the plasmid-mediated new extended-spectrum beta-lactamase TEM-27, was involved in a nosocomial outbreak (8 patients) at the Pediatric Cardiology Department of the Ramón y Cajal Hospital in Madrid. Genomic DNA polymorphism analysis, using an arbitrarily primed polymerase chain reaction, demonstrated the clonal nature of all Salmonella isolates. The plasmid encoding TEM-27 (pJMM1) was characterized by EcoRI, SacI, and BglI restriction. An identical restriction pattern was found in plasmid from all S. enterica strains. Possible in vivo intergeneric plasmid spread was suggested by the identification of an identical plasmid in Escherichia coli RYC5H and Enterobacter cloacae RYC39737 strains isolated during the outbreak. Results indicate that this outbreak involved dissemination of a single ceftazidime-resistant Salmonella strain and the spread of a single TEM-27-encoding plasmid among different Enterobacteriaceae.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Salmonella
/
Infecções por Salmonella
/
Beta-Lactamases
/
Infecção Hospitalar
Limite:
Child, preschool
/
Humans
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Infant
/
Newborn
Idioma:
En
Revista:
J Infect Dis
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Espanha