The terminal protein of the linear DNA plasmid pGKL2 shares an N-terminal domain of the plasmid-encoded DNA polymerase.
Yeast
; 12(3): 241-6, 1996 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-8904336
ABSTRACT
The 36K protein attached at the 5' end of the linear DNA plasmid pGKL2 from the yeast Kluyveromyces lactis was first purified and characterized. The terminal protein was purified from cells (1 kg wet weight) by ammonium sulphate precipitation and two rounds of centrifugation to equilibrium in CsCl gradients. The pGKL2 was present only in the post-microsomal supernatant. Approximately 10 mg of the purified pGKL2 was recovered and digested with DNase I. The terminal protein (final ca. 0 center dot 8 mg) was homogeneous by electrophoresis and we determined the N-terminal amino acid sequence up to ten residues, showing that it existed in the cryptic N-terminal domain of pGKL2-ORF2 (DNA polymerase) sequence.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Kluyveromyces
/
DNA Polimerase Dirigida por DNA
Idioma:
En
Revista:
Yeast
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Japão