Leishmania donovani: metabolite mapping of promastigotes using proton nuclear magnetic resonance spectroscopy.
Mol Cell Biochem
; 162(1): 17-22, 1996 Sep 06.
Article
em En
| MEDLINE
| ID: mdl-8905621
ABSTRACT
Proton nuclear magnetic resonance spectroscopy was used for studying the intracellular metabolite profile of promastigotes of Leishmania donovani. The major intracellular metabolites observed in the promastigotes were acetate, alanine, succinate, glycine, alpha-glycerophosphorylcholine, acetoacetate, arginine and ethanol. A comparative study of the intracellular metabolite profile of promastigotes of different strains of L. donovani showed that, all the major intracellular metabolites were present in promastigotes of different strains. A quantitative estimation of metabolites showed a strain specific (Finger print) metabolite profile which can be used for strain/species identification/differentiation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Leishmania donovani
Limite:
Animals
Idioma:
En
Revista:
Mol Cell Biochem
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Índia