The role of protein tyrosine phosphatase SHP-1 in the regulation of IFN-gamma signaling in neural cells.
J Immunol
; 157(11): 5139-44, 1996 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-8943425
ABSTRACT
The role for protein tyrosine phosphatase SHP-1 in controlling signal transduction by IFN-gamma in astrocytes was studied. IFN-gamma induced the gamma-activated factor (GAF) within 30 min and GAF subsequently declined by 8 h after treatment. However, treatment with IFN-gamma in the presence of protein tyrosine phosphatase inhibitor vanadate blocked the decrease in GAF activity. The increased stability of GAF in vanadate-treated cultures was similarly observed in astrocytes of motheaten mice, which specifically lack the protein tyrosine phosphatase SHP-1. Prolongation of GAF activity coincided with increased expression of the IFN-inducible transcription factor, IFN-regulatory factor-1 (IRF-1). Increased IRF-1 was coincident with increased expression of MHC class I molecules in astrocytes in accordance with the activity of IRF-1 in the promoter region. These data implicate an important role for protein tyrosine phosphatases, including SHP-1, in the regulation of IFN-gamma-signaling and IFN-gamma-inducible genes in neural cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transdução de Sinais
/
Astrócitos
/
Interferon gama
/
Proteínas Tirosina Fosfatases
Limite:
Animals
Idioma:
En
Revista:
J Immunol
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Estados Unidos