Lymphocytes subsets in normal individuals: analysis by four color immunofluorescence and flow cytometry on whole blood.
Tissue Antigens
; 48(4 Pt 1): 312-8, 1996 Oct.
Article
em En
| MEDLINE
| ID: mdl-8946685
ABSTRACT
In order to precisely define human lymphocytes subsets, we used four color immunofluorescence analysis and flow cytometry. We report here the results of systematic studies performed on whole blood from 20 normal volunteers. This was performed using monoclonal antibodies recognizing 18 different CD molecules and analyzed here into 14 different 4-color immunofluorescence combinations. This showed that among T cells, the two major CD3+CD8+ and CD3+CD8- subsets differed greatly in the percentage of cells expressing CD26, CD27, CD28, CD38, CD45RA, CD45RO, CD57, S6F1, BY55, CD101 and their respective combination representing an array of subsets. Furthermore the minor NK subsets, i.e. CD2+CD3-CD8+, CD2-CD3-CD8+, CD2+CD3-CD8-, CD2-CD3-CD8- also differed in percentages of cells expressing CD16, CD56, CD57, S6F1, BY55 and their combinations.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Antígenos CD
/
Subpopulações de Linfócitos
/
Técnica Direta de Fluorescência para Anticorpo
/
Citometria de Fluxo
Limite:
Humans
Idioma:
En
Revista:
Tissue Antigens
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
França