Subcellular distribution of glycosylphosphatidylinositol-specific phospholipase D in rat liver.
Biochem J
; 320 ( Pt 1): 315-9, 1996 Nov 15.
Article
em En
| MEDLINE
| ID: mdl-8947503
ABSTRACT
Glycosylphosphatidylinositol (GPI)-hydrolysing enzymes have been described in many mammalian tissues and body fluids; however, their site(s) of action and in vivo functions have remained unclear. In order to identify a possible intracellular site of GPI hydrolysis, we studied the subcellular distribution of GPI-hydrolysing activity in rat liver. We found that purified fractions from rat liver hydrolysed the GPI moieties of two GPI-anchored proteins with the specificity of a phospholipase D. This GPI-specific phospholipase D (GPI-PLD) activity was found to be highly enriched in a lysosomal fraction and showed a similar intracellular distribution to that of typical lysosomal enzymes. Our results indicate that lysosomes may represent a possible intracellular site of GPI-PLD action.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfolipase D
/
Frações Subcelulares
/
Fígado
Limite:
Animals
Idioma:
En
Revista:
Biochem J
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Suíça
País de publicação:
ENGLAND
/
ESCOCIA
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GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM