Stimulation of glutathione synthesis of in vitro matured bovine oocytes and its effect on embryo development and freezability.
Mol Reprod Dev
; 45(4): 451-7, 1996 Dec.
Article
em En
| MEDLINE
| ID: mdl-8956283
ABSTRACT
Glutathione (GSH) has been shown to play an important role in embryo development. In a previous study, we demonstrated that cysteamine supplementation of in vitro maturation (IVM) medium increased the intracellular GSH content in bovine oocytes and improved subsequent embryo development to the blastocyst stage. The present study was carried out to evaluate the effect of inhibition by buthionine sulfoximide (BSO) of GSH synthesis during IVM in the presence of cysteamine, on subsequent embryo development, and the effect of cysteamine during IVM on the survival of blastocysts following freezing. The effect of beta-mercaptoethanol and cysteine added to the maturation medium on GSH levels in bovine oocytes, as well as the effect of these compounds on de novo GSH synthesis by oocytes during in vitro maturation, was also studied. The inhibitory effect of BSO during in vitro maturation on GSH synthesis was also evaluated. Evidence was found confirming that GSH synthesis occurs intracellularly during IVM of oocytes and is stimulated by cysteamine, beta-mercaptoethanol and cysteine. Moreover, the present results suggest that the increase in the rate of embryo development exerted by cysteamine, when present during IVM, was due to its stimulatory effect on GSH synthesis. This increase in GSH levels during IVM improves embryo development and quality, producing more embryos reaching the blastocyst stage on day 6, those most suitable for freezing.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Criopreservação
/
Butionina Sulfoximina
/
Cisteamina
/
Cisteína
/
Desenvolvimento Embrionário e Fetal
/
Glutationa
/
Mercaptoetanol
/
Antimetabólitos
Limite:
Animals
Idioma:
En
Revista:
Mol Reprod Dev
Assunto da revista:
BIOLOGIA MOLECULAR
/
MEDICINA REPRODUTIVA
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Argentina