Role of the locus coeruleus in the sleep rebound following two different sleep deprivation methods in the rat.

ABSTRACT

The aim of the present study was to assess the involvement of the locus coeruleus in the paradoxical sleep rebound following sleep deprivation in the rat. Animals were sleep-deprived for 10 h before, and after, specific N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP-4) lesioning of the noradrenergic-locus coeruleus system. Sleep deprivation was produced using either an instrumental (water tank) or pharmacological (methylamphetamine) method. After lesioning, the rats submitted to the instrumental method showed a significant decrease in the paradoxical and slow-wave sleep rebounds (-54% and -78%, respectively), while animals receiving metamphetamine did not. Our results suggest that the noradrenergic system of the locus coeruleus is a relevant component of the sleep rebound mechanisms. However, the extent of involvement is dependent on the sleep deprivation method used.