Processing of semen in an antibiotic-rich culture medium to minimize microbial presence during in vitro fertilization.
Fertil Steril
; 67(1): 98-103, 1997 Jan.
Article
em En
| MEDLINE
| ID: mdl-8986691
ABSTRACT
OBJECTIVE:
To identify and determine the prevalence of microorganisms in preprocessed and postprocessed semen in an IVF-ET program.DESIGN:
Prospective study.SETTING:
University Teaching Hospital. PATIENT(S) Seventy-four men undergoing preprogram evaluation, each producing two semen samples. INTERVENTION(S) Semen processing with a wash and swim-up technique in a penicillin- and streptomycin-rich medium. MAIN OUTCOME MEASURE(S) The identity and prevalence of seminal microorganisms before and after processing. RESULT(S) Sixty-three percent of individual unprocessed semen samples grew microorganisms, the majority of which were nonpathogenic. Thirty-three men (44.6%) had microbes identified in repeat samples, four had identical organisms each time. Twenty (27%) had positive cultures in one sample, negative in the other. Twenty-one (28.4%) had consistently sterile semen. After seminal processing, the recovery rate for microbes was 5%. Microbial presence after processing did not correlate with either the sperm swim-up concentration or the initial microbial concentration. CONCLUSION(S) Bacteriospermia is common. The microorganisms found rarely are replicated and most likely represent contamination. Wash and swim-up semen preparation in an antibiotic rich culture medium effectively eliminates 95% of organisms.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sêmen
/
Bactérias
/
Fertilização in vitro
/
Antibacterianos
Tipo de estudo:
Observational_studies
/
Risk_factors_studies
Limite:
Humans
/
Male
Idioma:
En
Revista:
Fertil Steril
Ano de publicação:
1997
Tipo de documento:
Article
País de publicação:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA