Automated high-performance liquid chromatographic separation with spectrofluorometric detection of a malondialdehyde-thiobarbituric acid adduct in plasma.
J Chromatogr A
; 729(1-2): 207-10, 1996 Apr 05.
Article
em En
| MEDLINE
| ID: mdl-9004941
ABSTRACT
An automated HPLC separation method for the detection of malondialdehyde (MDA) in plasma samples of a healthy population is described. The procedure involves a first step of acidic hydrolysis and complex formation with thiobarbituric acid (TBA), then a protein precipitation step, and finally the separation of TBA-MDA adducts through a complete HPLC apparatus with spectrofluorometric quantification. This procedure is very useful for a routine laboratory because of its rapidity (tR = 1.7 min), simplicity and applicability, and the method gives very good results with respect to linearity (0.1-12 mumol/l), precision (within-assay C.V.% 8, between-assay C.V.% 10) and accuracy (recovery average 91.66%).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Substâncias Reativas com Ácido Tiobarbitúrico
Tipo de estudo:
Diagnostic_studies
Limite:
Adolescent
/
Adult
/
Aged
/
Humans
/
Middle aged
Idioma:
En
Revista:
J Chromatogr A
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Itália