Structural and serological characterisation of the O-specific polysaccharide from lipopolysaccharide of Acinetobacter calcoaceticus strain 7 (DNA group 1).
Eur J Biochem
; 243(1-2): 167-73, 1997 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-9030736
ABSTRACT
S-form lipopolysaccharide was isolated by phenol/water extraction from a strain of Acinetobacter calcoaceticus (DNA group 1 ). The structure of the O-antigenic polysaccharide was determined by compositional analysis and NMR spectroscopy of the de-O-acylated lipopolysaccharide. The isolated polysaccharide obtained after hydrolysis of lipopolysaccharide in 0.01 M trifluoroacetic acid has the following structure [STRUCTURE IN TEXT] in which Pyr is pyruvate. The O-acetyl substitution of D-Gal was non-stoichiometric. The O-antigen was specifically recognised in western blots by polyclonal rabbit antisera.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Lipopolissacarídeos
/
Acinetobacter calcoaceticus
/
Antígenos de Bactérias
Limite:
Animals
Idioma:
En
Revista:
Eur J Biochem
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Alemanha