The C-terminus of glycosylphosphatidylinositol-specific phospholipase D is essential for biological activity.
Biochim Biophys Acta
; 1355(2): 107-13, 1997 Feb 04.
Article
em En
| MEDLINE
| ID: mdl-9042330
ABSTRACT
Glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD) (EC 3.1.4.50) from mammalian serum is a 115 kDa glycoprotein consisting of 816 amino acids. We found that C-terminal deletions of only two to five amino acids reduced GPI-PLD enzymatic activity by roughly 70% as compared to wild-type protein. C-terminal deletions of more than five amino acids resulted in a complete loss of GPI-PLD enzymatic activity. Point mutations at position 811 indicate that Tyr-811 may play a major role in maintaining the biological activity of GPI-PLD.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfolipase D
Limite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Suíça