Albumin standards and the measurement of serum albumin with bromcresol green. 1971.
Clin Chim Acta
; 258(1): 21-30, 1997 Feb 03.
Article
em En
| MEDLINE
| ID: mdl-9049440
ABSTRACT
A rapid and reliable method for measuring serum albumin employing bromcresol green is described. The addition of albumin to a solution of bromcresol green in a 0.075 M succinate buffer pH 4.20 results in an increase in absorbance at 628 nm. The absorbance-concentration relationship is linear for samples containing up to 6 g/dl albumin. Bilirubin, moderate lipemia, and salicylate do not interfere with the analysis. The use of nonionic surfactant (Brij-35) reduces the absorbance of the blank, prevents turbidity and provides linearity. The results by this method agree very well with those obtained by electrophoresis and salt fractionation. The method is simple, it has excellent precision and the reagents are stable. A protein standard is introduced which can be employed for both the total serum proteins and albumin determinations.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Análise Química do Sangue
/
Albumina Sérica
Limite:
Humans
Idioma:
En
Revista:
Clin Chim Acta
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Estados Unidos