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Chromosomal changes observed in immortalized human keratinocytes transformed by ionizing radiation.
Hukku, B; Thraves, P; Dritschilo, A; Rhim, J S.
Afiliação
  • Hukku B; Children's Hospital of Michigan, Department of Pediatrics, Detroit 48201, USA.
Cancer Genet Cytogenet ; 93(2): 125-39, 1997 Feb.
Article em En | MEDLINE | ID: mdl-9078297
Human epithelial cancer cells were induced by concerted action of DNA tumor virus and X-ray radiation. Treatment of nontumorigenic early passage AD12-SV40 immortalized epithelial cells (RHEK-1) at passage 23 with radiation, resulted in further changes in their growth properties. One day old cultures of these RHEK-1 cells were irradiated with graded doses of X-rays (0, 2, 4, 6, and 8 Gy i.e. RHEK-1, RHEK-1/200R, RHEK-1/400R, RHEK-1/600R, and RHEK-1/800R). Morphologic alterations, the ability to grow in soft agar, and to form rapidly-growing squamous cell carcinomas in nude mice were concomitantly acquired properties of the radiation transformed cell lines RHEK-1/200R and RHEK-1/ 400R. On the basis of commonality in having addition of some extra material in chromosome 11 in the region between q14/q22 in all tumorigenic cell lines RHEK-1/200R and RHEK-1/400R, and deletion of the same region in nontumorigenic irradiated cell lines-RHEK-1/600R and RHEK-1/800R, it is deduced this region may have some important oncogene/s or other gene/s that play an important role in tumorigenesis. When compared to squamous cell carcinoma data, the duplication observed in the present study is also observed in 28 to 38% of head and neck and also in 25% of cases of untreated malignant lesions of oral squamous cell carcinoma. Thus, this study shows the correlation between in vitro induced squamous cell carcinoma to in vivo tumors.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carcinoma de Células Escamosas / Queratinócitos / Aberrações Cromossômicas Limite: Animals / Humans Idioma: En Revista: Cancer Genet Cytogenet Ano de publicação: 1997 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carcinoma de Células Escamosas / Queratinócitos / Aberrações Cromossômicas Limite: Animals / Humans Idioma: En Revista: Cancer Genet Cytogenet Ano de publicação: 1997 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos