The angiogenesis inhibitor TNP-470 reduces the growth rate of human neuroblastoma in nude rats.
Pediatr Res
; 41(3): 327-33, 1997 Mar.
Article
em En
| MEDLINE
| ID: mdl-9078530
ABSTRACT
A new animal experimental model of human neuroblastoma is described. The model involves xenotransplantation of a poorly differentiated human neuroblastoma cell line (SH-SY5Y) to the subcutaneous tissue in the hind leg of nude rats (WAG mu/rnu). Injection of 20 million cells suspended in 0.2 mL of medium in each hind leg yielded an 89% tumor take (41/46) in 23 nude rats. Tumor take was evident after 2 wk. The tumors grew exponentially and reached a volume of 5.2 +/- 1.6 mL 4 wk after transplantation. The tumor cells retained their morphologic phenotype at the ultrastructural level after transplantation and were immunohistochemically positive for neuron-specific enolase and for chromogranins A and B. Subcutaneous injections of the angiogenesis inhibitor TNP-470 (10 mg/kg of body weight) every other day gave a treated/control quotient for mean tumor volume of 0.34 after 12 d of treatment. This implies that angiogenesis inhibition may be of value as a complement to chemotherapy in the treatment of human neuroblastoma. The presented animal experimental model is designed for investigations of the effects of chemotherapy, angiogenesis inhibitors, radiotherapy, and/or surgery on the growth rate of human neuroblastoma.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sesquiterpenos
/
Doenças do Sistema Nervoso Autônomo
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Antibióticos Antineoplásicos
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Neovascularização Patológica
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Neuroblastoma
Limite:
Animals
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Female
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Humans
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Male
Idioma:
En
Revista:
Pediatr Res
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Suécia