Presenilin-1 is processed into two major cleavage products in neuronal cell lines.
Neurodegeneration
; 5(4): 293-8, 1996 Dec.
Article
em En
| MEDLINE
| ID: mdl-9117540
ABSTRACT
Presenilin-1 (PS-1) has been identified as the protein encoded by the chromosome 14 locus that, when mutated, leads to familial Alzheimer's disease (FAD). Using PS-1 transfected SHSY5Y neuroblastoma cells, we have demonstrated by immunodetection, using polyclonal antibodies, that PS-1 is processed to give two fragments an N-terminal 28 kDa fragment, and a C-terminal 18 kDa fragment. In a number of non-transfected cell types, most PS-1 is detected as the cleaved products. The molecular weights of the PS-1 cleavage products suggest that the cleavage point will most probably be within a region of the hydrophilic loop domain coded for by either exon 8 or 9 of the PS-1 gene. The clustering of FAD mutations within exon 8 strongly suggests that it encodes a key functional domain. It seems likely that the cleavage of PS-1 is crucial to some aspect of its functionality. An understanding of this process will give insights into the pathology of AD, and may offer new opportunities for therapeutic intervention.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Processamento de Proteína Pós-Traducional
/
Proteínas de Membrana
/
Neurônios
Idioma:
En
Revista:
Neurodegeneration
Assunto da revista:
NEUROLOGIA
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Reino Unido